Composite

Part:BBa_K4209013:Design

Designed by: Marta Marangoni   Group: iGEM22_UNILausanne   (2022-10-09)


constitutive FitD


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 57
    Illegal EcoRI site found at 5106
    Illegal PstI site found at 564
    Illegal PstI site found at 618
    Illegal PstI site found at 1821
    Illegal PstI site found at 2607
    Illegal PstI site found at 7851
    Illegal PstI site found at 8406
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 57
    Illegal EcoRI site found at 5106
    Illegal PstI site found at 564
    Illegal PstI site found at 618
    Illegal PstI site found at 1821
    Illegal PstI site found at 2607
    Illegal PstI site found at 7851
    Illegal PstI site found at 8406
    Illegal NotI site found at 8760
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 57
    Illegal EcoRI site found at 5106
    Illegal XhoI site found at 6183
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 57
    Illegal EcoRI site found at 5106
    Illegal PstI site found at 564
    Illegal PstI site found at 618
    Illegal PstI site found at 1821
    Illegal PstI site found at 2607
    Illegal PstI site found at 7851
    Illegal PstI site found at 8406
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 57
    Illegal EcoRI site found at 5106
    Illegal PstI site found at 564
    Illegal PstI site found at 618
    Illegal PstI site found at 1821
    Illegal PstI site found at 2607
    Illegal PstI site found at 7851
    Illegal PstI site found at 8406
    Illegal NgoMIV site found at 506
    Illegal NgoMIV site found at 850
    Illegal NgoMIV site found at 1543
    Illegal NgoMIV site found at 2039
    Illegal NgoMIV site found at 2829
    Illegal NgoMIV site found at 2945
    Illegal NgoMIV site found at 3053
    Illegal AgeI site found at 3788
    Illegal AgeI site found at 5937
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 281
    Illegal BsaI site found at 1148
    Illegal BsaI.rc site found at 1470
    Illegal BsaI.rc site found at 4122


Design Notes

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Source

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References